Abstract:
DNA barcoding is a widely utilized molecular-based
identification of species and taxonomic resolutions.
Until recently, Rhamdia voulezi and Rhamdia
branneri were considered species synonyms of
Rhamdia
quelen; however, morphological
and cytogenetic analyses have suggested the validity
of distinct species. Due to the absence of molecular
taxonomy of R. voulezi and R. branneri,
the objective of this study was to test its validity
through traditional DNA barcoding and the GMYC (General
Mixed Yule Coalescent) COI-based analyses in 19 specimens
from the Iguaçu River Basin. In both methodologies,
three MOTUs (Molecular Operational Taxonomic Units)
were identified based on the estimated optimum threshold
(OT = 0.77). The average inter-MOTU distance (NJ,
K2P) between R. branneri and R. voulezi
was 1.4%, and 0% intra-MOTU distance in both species.
The two species identified as R. branneri
and R. voulezi showed correspondence with
taxonomic and morphological identifications. With
regard to R. quelen, the average intra-MOTU
distance was greater than OT (2.7%), indicating that
this species can be formed by different MOTUs. We
suggest that molecular and taxonomic studies should
be employed concurrently in R. quelen, to
prevent contamination of wild species by hybridizations
(Ribolli, J. et al. 2017). Etymology:
The specific name branneri: In honour of
American geologist John Casper Branner (1950–1922),
who “kindly assisted” Haseman at the beginning
of his “long journey” to central South
America. Remarks:
This species can still be found on some online searches
as Rhamdia quelen with R. branneri
being a synonym off.
Common
Name:
None
Synonyms:
None
Family:
Heptapteridae
Distribution:
South America:
Iguaçu River basin (Brazil and Argentina).
Type locality: Creek of the Rio Iguaçú
near Serrinha, Paraná, Brazil.
Rhamdia branneri Iguaçu River Basin, Paraná State,
Brazil
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